subcellular fractionation

Joel A. Kreps jak8 at po.CWRU.Edu
Fri Apr 17 14:02:05 EST 1992


Dear Network,		April 17,

I am interested in finding out what methods people are using to
produce subcellular fractions (nuclear vs cytosol) for use in 
western blots with antibody staining to determine if a particular
protein is localized to the nucleus or cytosol.  I am working with
Arabidopsis but am interested in both plant and non-plant methods.  
I have several methods already but they are rather old and I would
like to know what is most popular now.  Thanks.

Joel A. Kreps



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