mcgraw%gandal.dnet at SERVER.UGA.EDU mcgraw%gandal.dnet at SERVER.UGA.EDU
Thu Apr 9 09:26:23 EST 1992

Hello Netters,

A recent short article in NAR (Ponce, M.R., and Nicol, J.L., 1992, Vol. 20, 
p.623) describes conditions for PCR amplification of long (>6 kb) DNA
fragments.  The 10X buffer contains 1% Thesit. The SIGMA catalog says
Thesit is a trade name for polyoxyethylene 9-lauryl ether.

Can anyone out there tell us more about Thesit in PCR?  If it's good, why
is it good?  Where did you get it?  Any comments appreciated.


				Al McGraw
				University of Georgia

More information about the Methods mailing list