Bind silane protocols for seq gels

Jack Kramer kramer at news.miami.edu
Thu Apr 9 07:34:27 EST 1992


In article <1992Feb25.214601.21945 at acsu.buffalo.edu> camlau at ubvmsb.cc.buffalo.edu (B.J.) writes:
>In article <1992Feb25.141537.935 at uwovax.uwo.ca>, 37_527 at uwovax.uwo.ca (Gregory A. Denomme) writes...
>>  I would like to obtain receipes for coating the outer plates for sequencing
>>gels using BDH's Silane A-174 as the 'bind Silane'.  I am having some trouble
>>at the point of separating the plates and maybe a new protocol would help.
>
>Our lab uses PAM to help gels unstick.  That's right, PAM, the vegetable
>spray shortening.  Seems to work wonders.  Just spray on, wipe down excess.
>

Has anyone tried this for gels for IEF of proteins?  Sure is a lot
cheaper than the commercial repel silanes.

Is there a similar alternative for the "bind" side plate?

jack kramer
Univ of Fla
Tropical Reearch and Education Center
kramer at molbio.med.miami.edu



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