filamentous phage RF

Rueker ruker at
Tue Aug 4 10:12:51 EST 1992

Hi netters,

I have a problem with the preparation of replicative
form of fuse5 (a derivative of the filamentous phage fd-tet. What happens 
is that after the bacteria have grown under selection pressure, 
(meaning the phage must be there) only minute amounts of DNA are 
recovered from 0.5 l of culture, just enough to get 
ca. 100 colonies after electroporation. The protocol that is used is
exactly what George Smith writes in his manuscript. With other, similar
phages, this problem does not occur.

Does anybody have a similar problem or a suggestion?  

Florian Rueker
University of Agriculture
Institute of Applied Microbiology
Nussdorfer Laende 11
A - 1190 Vienna, Austria

Tel: 0043/1/36 92 924 458

Fax: 0043/1/36 92 924 400

e-mail: ruker at 

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