Nucleic Acid Precipitation

George W Chacko gchacko at magnus.acs.ohio-state.edu
Fri Aug 28 13:48:40 EST 1992


What are your thoughts on the relative merits of

a-	isopropanol
	
b-	95 % ethanol

c-	99% ethanol

when used to precipitate RNA, DNA and synthetic oligonucleotides out
of solution? I found in ine case that 95% ethanol did not substitute
efficiently for isopropanol when precipitating RNA but I did that
experiment only once.

George



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