PBR322 - maximizing yields

[Steve L. Moseley]

My experience with chloramphenicol amplification was that the earlier in log 
phase you add the chloramphenicol, the better. I recall adding it at about 
0.5 OD 540 for the best yields. Also, I seemed to get much improved yields 
with normal aeration. Contrary to folklore, the fragility of the cells didn'
t seem to be a problem.
__________________________________________________________________________
Steve L. Moseley                                  moseley at u.washington.edu
Microbiology SC-42                                Phone: (206) 543-2820
University of Washington                          FAX:   (206) 543-8297
Seattle, WA 98195