PBR322 - maximizing yields
Steve L. Moseley
moseley at u.washington.edu
Fri Aug 28 12:09:21 EST 1992
My experience with chloramphenicol amplification was that the earlier in log
phase you add the chloramphenicol, the better. I recall adding it at about
0.5 OD 540 for the best yields. Also, I seemed to get much improved yields
with normal aeration. Contrary to folklore, the fragility of the cells didn'
t seem to be a problem.
Steve L. Moseley moseley at u.washington.edu
Microbiology SC-42 Phone: (206) 543-2820
University of Washington FAX: (206) 543-8297
Seattle, WA 98195
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