polysaccharides in DNA extractions

Bill Melchior, NCTR/FDA WMELCHIOR at NTET.NCTR.FDA.GOV
Mon Aug 24 14:50:03 EST 1992


>DNA extractions for cacoa ( and some other tropical plants) are
>extremely difficult due to the co precipitation of mucus with the
>DNA.

Have you tried the high salt precipitation method of Fang, Hammar, & Grumet,
BioTechniques 13 #1, 52-56 (July 1992)?

They add NaCl to a concentration of 0.5 M to 3 M, which they claim leads
to precipitation of most of the polysaccharides.  See the paper for more
detail and hints.
________________________________________________________________________________
The opinions stated are mine, not those of NCTR or its sponsoring organizations.

Bill Melchior                                ||   "You have lawyers the way
National Center for Toxicological Research   ||    other people have mice."
Jefferson, AR  72079                         ||
(501) 543-7206                               ||   -Kenneth Duncan, English
                                             ||    Health & Safety Executive,
WMELCHIOR at NTDOC.NCTR.FDA.GOV                 ||    to US regulators



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