polysaccharides in DNA extractions

Mon Aug 24 14:50:03 EST 1992

>DNA extractions for cacoa ( and some other tropical plants) are
>extremely difficult due to the co precipitation of mucus with the

Have you tried the high salt precipitation method of Fang, Hammar, & Grumet,
BioTechniques 13 #1, 52-56 (July 1992)?

They add NaCl to a concentration of 0.5 M to 3 M, which they claim leads
to precipitation of most of the polysaccharides.  See the paper for more
detail and hints.
The opinions stated are mine, not those of NCTR or its sponsoring organizations.

Bill Melchior                                ||   "You have lawyers the way
National Center for Toxicological Research   ||    other people have mice."
Jefferson, AR  72079                         ||
(501) 543-7206                               ||   -Kenneth Duncan, English
                                             ||    Health & Safety Executive,
WMELCHIOR at NTDOC.NCTR.FDA.GOV                 ||    to US regulators

More information about the Methods mailing list