WAGNERF at BRUNB.BITNET
Fri Aug 21 10:33:49 EST 1992
100 - 150 pMol is a standard for most protein sequencing centers,
and the reason for that is to increase the probability of obtaining
a N-terminal sequence as long as possible.
If your protein is very hard to obtain, and you do not need more
than 10 residues from the N-terminal, it is possible to try sequencing
at the 1 pMol range.
Dr. Wagner Fontes
Brasilian Center of Protein Sequencing
Biochemistry-Protein Chemistry Laboratory
University of Brasilia
More information about the Methods