Protein microsequencing

DMEIER at mis.mcw.edu DMEIER at mis.mcw.edu
Tue Aug 18 19:59:14 EST 1992


Hello All,
  To sequence the amino terminal end of a peptide, a facility in Wisconsin 
requires 150 pmol of protein. If the protein is approx. 60,000 daltons, 
then sequencing requires 3 micrograms of pure protein.  Is that typical 
for protein sequencing facilities?  I'd appreciate your replies.

Dan Meier



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