Superbroth and plasmid preps?
Andrew F. Cockburn
afc at gnv.ifas.ufl.edu
Tue Aug 18 13:21:09 EST 1992
I saw an article a few weeks ago in a biotech company's newletter
touting the advantages of growing plasmids in rich media. The
newletter went the way of all such things, but now I wonder if I
should have kept it.
Does anyone have experience using rich media (eg 2x or 3x L broth)
for growing plasmids in E. coli? Do the specific vector and host
matter? Are the yields high enough to justify switching from L
broth? What is your media recipe? Do you use the same plasmid
prep as before?
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