> I have been wondering if any of the protein A and protein G alternatives bind
> to chicken immunoglobulins with good affinity? I have been trying to develop
> an immunoprecipitation method using chicken serum. The anti-chicken
> immunoglobulin that I have been using seems to precipitate some other proteins
> as well interfering with my results.
Have you considered other alternatives like the immobilized anti-chicken
immunoglobulin. As far as I knew, one company used to make this preparation
i.e., latex sphere bound anti-chicken immunoglobulin. I think this method
may increase the specificity of your assay and thus more reliable results.
The place I used to buy these from is Kirkegaard and Perry Laboratories
It is in Gaithersburg Maryland. Tel: (800) 638-3167.
Hope this helps
University of Pittsburgh