RNA ligase & single-stranded DNA

Eric C. Kofoid kofoid at bioscience.utah.edu
Fri Aug 14 12:04:15 EST 1992


I am going ligate oligonucleotides into linearized single-stranded
plasmid 
DNA using RNA ligase. When I last did this (years ago), it was very 
inefficient. I would appreciate any favorite enhancements or references
to 
this method. 

 __________________________________________________________________
|  Eric Kofoid                         Dept.Biology, U. of Utah    |
|  "Being certain of the conclusion assists in finding the proof"  |
|                                      Galileo, 17th century       |
|__________________________________________________________________|



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