Lysate RNAase Protection Assay

kim at m44.unm.edu kim at m44.unm.edu
Wed Aug 12 13:13:33 EST 1992


Hello:

Has anyone tried out the "Lysate RNAase Protection Assay" of Haines and
Gillespie (BioTechniques June, 1992)?

Does it really work?  Is it comparable in sensitivity to RPA using purified
RNA?  Is there a problem with the fact that there seems to be no step for
denaturing the probe/target molecules by heating prior to hybridization?

I understand there is a kit available.  Is it substantially different from the
procedure described in the paper?

Thanks.

Daniel Kim
KIM at FLOVAX.LANL.GOV



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