Ligation in melted Nusieve

Andy Phillips PHILLIPSA at UK.AC.AFRC.LARS
Wed Aug 12 09:54:00 EST 1992


The booklet that comes with Nusieve GTG agarose claims that ligation
of isolated fragment to vector can be carried out in melted Nusieve.
Some guys here have tried this with plasmid vectors and it works fine.
My question is: will this technique work with lambda insertion vectors
(eg ZapII), or will the agarose interfere with ligation and/or
packaging (I could test this myself, but Gigapack Gold isn't
the cheapest ingredient ever).
Thanks
Andy Phillips
Cell Biology Dept, LARS, Bristol, UK



More information about the Methods mailing list