Cs gradients (from CHEESE thread)
Bill Melchior, NCTR/FDA
wmelchior at NTBTOX.NCTR.FDA.GOV
Wed Aug 12 08:20:53 EST 1992
>I'm still trying to figure out why anyone still uses Cesium
>gradients. As far as I can tell, they add a couple of days and
>a lot of trouble for very little gain. Yet I still see a lot
>of labs doing them.
When I want VERY clean plasmid, I use the Cs/ethidium method of Garger
et al (BBRC 117, 835-842, 1983). It takes one day -- I spin for 4 3/4 hr.
The opinions stated are mine, not those of NCTR or its sponsoring organizations.
Bill (Cheesit) Melchior || "You have lawyers the way
National Center for Toxicological Research || other people have mice."
Jefferson, AR 72079 ||
(501) 543-7206 || -Kenneth Duncan, English
|| Health & Safety Executive,
WMELCHIOR at NTDOC.NCTR.FDA.GOV || to US regulators
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