Two Sequencing questions:
zsha at iastate.edu
Mon Aug 10 12:55:34 EST 1992
In <1992Aug10.154723.23852 at magnus.acs.ohio-state.edu> rrumpf at magnus.acs.ohio-state.edu (Robert Rumpf) writes:
>I have two questions regarding sequencing protocols:
>1. Our 35-S gels are still sticky after drying; the saran wrap sticks to them
>and is difficult to remove. Why? We also noticed that removing the saran wrap
>and placing film directly on the gels apparently causes some damage to the
>film from the moisture or urea in the gel...
>2. Anyone have any experience with 33-P yet? We would like to know if the
>energy from this isotope is sufficient to pass through saran wrap so we can
>expose films without taking the wrap off.
You mean 32-P? The answer is yes. Even with 35-S sequencing gel we exposure the dried gel without removing the saran wrap. The results are satisfied if you labeling reaction is good.
>Any help will be greatly appreciated...
Zhengyu Sha l 2188 Molecular Biology Building l
Phone No. (515)294-4210 l ISU, Ames,Iowa 50011 l
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