Methylase minus strain

Tony Weiss weiss at angis.su.oz.au
Mon Aug 10 00:28:19 EST 1992


Does anyone know of I good methylase deficient (mk-) strain of E. coli for
cloning? Most of our work involves methylase + strains, and we've lost a
restriction site! I was thinking of NM522 but have heard that it happily
recombines cloned DNA, but this may be an urban myth.

Any input would be appreciated.

Thanks

Tony Weiss
weiss at angis.su.oz.au
Dept Biochemistry  G08
University of Sydney
NSW  2006
Australia

"usual disclaimers apply"



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