dak at biosys.apldbio.COM
Thu Aug 6 13:55:11 EST 1992
Yes 4% NuSieve gels can be quite brittle. I handle all my agarose
gels using a long plastic spatula. They're cheap and effective. You
just have to be careful about sliding your gel onto the floor. For
really big gels I have used two spatulas, but the coordination
required seems to increase eponentialy.
Addition of agarose to NuSieve can increase tensile strength...just
have to play with the concentrations until you get one that works for
you molecules. I routinely run 3%NuSieve/1% agarose for separating
things in the 200 - 800 bp range.
DAK at apldbio.com
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