Fungal Electroporation

Jens Cavallius jxc15 at po.CWRU.Edu
Thu Aug 6 07:47:55 EST 1992

Are there anybody out there who can give us some advice on the settings
for eletroporation of yeast cells.  On cells that have been stored in 
i 1 M sorbitol in liq. nitrogen, we are getting either arcing or too
low time constant.  And those are the same cells that worked fine fresh,
before storage in liq. nitrogen.
(NB: the problem is NOT salt in the DNA solution, we use 1 microliter of TE).
Thanks					Jens Cavallius, PhD

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