Taq sticking to DNA

GENE SIMON HUH huh at wccf.mit.edu
Wed Aug 5 15:21:00 EST 1992


Dear fellow netters,

     I missed the discussion a while ago regarding how proteinase K treatment 
of PCR products renders them more clonable.  Could someone please email me the 
reference?  In a related vein, does this reference mention whether Taq sticks 
tenaciously enough to the ends of DNA even through a standard agarose gel?  If 
so, which way does the gelshift occur, if any shift at all?

Thanking you all in advance,

Gene Huh
huh at wccf.mit.edu    or    huh at mitwccf



More information about the Methods mailing list