Dear fellow netters,
I missed the discussion a while ago regarding how proteinase K treatment
of PCR products renders them more clonable. Could someone please email me the
reference? In a related vein, does this reference mention whether Taq sticks
tenaciously enough to the ends of DNA even through a standard agarose gel? If
so, which way does the gelshift occur, if any shift at all?
Thanking you all in advance,
huh at wccf.mit.edu or huh at mitwccf