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Taq sticking to DNA

GENE SIMON HUH huh at wccf.mit.edu
Wed Aug 5 15:21:00 EST 1992

Dear fellow netters,

     I missed the discussion a while ago regarding how proteinase K treatment 
of PCR products renders them more clonable.  Could someone please email me the 
reference?  In a related vein, does this reference mention whether Taq sticks 
tenaciously enough to the ends of DNA even through a standard agarose gel?  If 
so, which way does the gelshift occur, if any shift at all?

Thanking you all in advance,

Gene Huh
huh at wccf.mit.edu    or    huh at mitwccf

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