Inosine containing oligos

ww40 William_D_WARREN at UMAIL.UMD.EDU
Wed Aug 5 14:37:00 EST 1992


G'day all,

I'm contemplating incorporating inosine into an oligonucleotide
in place of a mixture of all four bases (since inosine will apparently
base pair equally well with A T G or C ).

My question is, how does the strength of I-A, I-G, I-T or I-C
bonds compare with A-T and G-C pairing ?

Also if one were to incorporate 4 inosines in a row would this
cause any conformational problems and disrupt the base pairing
of flanking residues ???

Any information or comments greatly appreciated.

Bill Warren
Center for Ag. Biotech
University of Maryland




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