Inosine containing oligos
William_D_WARREN at UMAIL.UMD.EDU
Wed Aug 5 14:37:00 EST 1992
I'm contemplating incorporating inosine into an oligonucleotide
in place of a mixture of all four bases (since inosine will apparently
base pair equally well with A T G or C ).
My question is, how does the strength of I-A, I-G, I-T or I-C
bonds compare with A-T and G-C pairing ?
Also if one were to incorporate 4 inosines in a row would this
cause any conformational problems and disrupt the base pairing
of flanking residues ???
Any information or comments greatly appreciated.
Center for Ag. Biotech
University of Maryland
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