Random priming advice needed

[David J. Meyer]

Use less template DNA (25 ng should be enough for all but the largest hyb volumes) and take good care of your Klenow! If 5 people in a lab each put the enzyme 
on ice for 15 minutes ("just a little while...") while they plan their experiments, it doesn't take long for the enzyme to die. Better still, make up your own 
kit; it won't necessarily work better, but will force you to really learn about 
what's going on.

Dave Meyer
djmeyer at ucdavis.edu
Mann Laboratory
Department of Vegetable Crops
University of California, Davis