Random priming advice needed

David J. Meyer ez005587 at chip.ucdavis.edu
Tue Dec 1 02:27:27 EST 1992


Use less template DNA (25 ng should be enough for all but the largest hyb volumes) and take good care of your Klenow! If 5 people in a lab each put the enzyme 
on ice for 15 minutes ("just a little while...") while they plan their experiments, it doesn't take long for the enzyme to die. Better still, make up your own 
kit; it won't necessarily work better, but will force you to really learn about 
what's going on.

Dave Meyer
djmeyer at ucdavis.edu
Mann Laboratory
Department of Vegetable Crops
University of California, Davis



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