guanidine life span

Loryn Sellner loryn at microbiol.uwa.oz.au
Wed Dec 2 00:38:21 EST 1992


I have just recently started using a guanidine thiocyanate extraction method 
for extracting viral RNA. I want to know how long the extraction solution 
can be stored for and under what conditions (i.e. frozen, at 4 C, etc.).
Any information from other people using this method would be greatly 
appreciated. The extraction solution contains 8M guanidine isothiocyanate, 
50mM Na citrate, 100 mM 2-ME, 1% sarkosyl, 1 ug/ml yeast tRNA.
Ta.

Loryn Sellner
Department of Microbiology



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