PEG and blunt end ligations

guo at mcclb0.med.nyu.edu guo at mcclb0.med.nyu.edu
Tue Dec 1 18:33:26 EST 1992


In article <1992Nov27.182237.8551 at magnus.acs.ohio-state.edu>, gchacko at magnus.acs.ohio-state.edu (George W Chacko) writes:
> I'm looking for a reference on the use of PEG in blunt end ligations.
> or if anyone has experience with the use of this reagent in ligations
> I'd appreciate a response.
> 
> Thanks
> 
> George
There are two papers which may be useful:

Zimmerrman,S. B. and Pheiffer,B. H.    Macromolecular crowding allows
blunt-end ligation by DNAligases from rat liver or E. coli.
PNAS, USA  80:5852-5856, (1983).

Teraoka and Tsukada. J. Biochem. (Tokyo) 101:225-231, (1987)

I hope this helps.

Sheng Guo
NYU Medical Center 



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