magic mp & sequenase

[Paul N Hengen]

wmelchior at ntet.nctr.fda.gov writes:

>> In advance of some experiments to be performed by a co-worker (and at
>> the request of my boss), I would like to find out if anyone has tried
>> using plasmid DNA recovered with Promega's Magic MiniPrep kit directly
>> for use with the Sequenase kit.

>I also am curious about this.  I was recently given some samples of the 
>Promega system, which claims to act "more effectively than silicon-based 
>media" but is used in a manner VERY similiar to powdered silica, except it 
>uses guanidine HCl instead of NaI.  (Both are chaotropic agents, so the 
>binding mechanism is probably the same.)...

What is the binding matrix of the Promega mini-prep column used in this kit?


Paul N. Hengen
National Cancer Institute
Frederick Cancer Research and Development Center
Frederick, Maryland 21702-1201 USA