magic mp & sequenase
Paul N Hengen
pnh at fcsparc6.ncifcrf.gov
Thu Dec 3 16:55:31 EST 1992
wmelchior at ntet.nctr.fda.gov writes:
>> In advance of some experiments to be performed by a co-worker (and at
>> the request of my boss), I would like to find out if anyone has tried
>> using plasmid DNA recovered with Promega's Magic MiniPrep kit directly
>> for use with the Sequenase kit.
>I also am curious about this. I was recently given some samples of the
>Promega system, which claims to act "more effectively than silicon-based
>media" but is used in a manner VERY similiar to powdered silica, except it
>uses guanidine HCl instead of NaI. (Both are chaotropic agents, so the
>binding mechanism is probably the same.)...
What is the binding matrix of the Promega mini-prep column used in this kit?
Paul N. Hengen
National Cancer Institute
Frederick Cancer Research and Development Center
Frederick, Maryland 21702-1201 USA
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