HighFive cells- Are they worth it?
Clifford J Beall
cbeall at magnus.acs.ohio-state.edu
Fri Dec 4 17:50:17 EST 1992
Dear net denizens,
I am expressing a secreted protein using baculovirus and Sf21 cells. I was
wondering if anyone would like to pass on experiences with Invitrogen's
HighFive cells. They are quite pricey, but they claim up to 25-fold higher
expression (I'm getting about 1 ug/ml without optimization). Also, if they
work well, is there a public source?
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