HighFive cells- Are they worth it?

Clifford J Beall cbeall at magnus.acs.ohio-state.edu
Fri Dec 4 17:50:17 EST 1992


Dear net denizens,

I am expressing a secreted protein using baculovirus and Sf21 cells.  I was 
wondering if anyone would like to pass on experiences with Invitrogen's 
HighFive cells.  They are quite pricey, but they claim up to 25-fold higher 
expression (I'm getting about 1 ug/ml without optimization).  Also, if they 
work well, is there a public source?



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