Vent of Pfu - which is more HiFi ?

Ashok Aiyar axa12 at po.cwru.edu
Fri Dec 4 17:05:46 EST 1992


In article <9212032143.AA07769 at neb.com> kucera at neb.com writes:

>>Has anyone out there compared the thermostable polymerases Vent and Pfu ?
>>Any comments you may have on the RELATIVE merits of one over the other would
>>be appreciated (eg length of synthesis products, stability, etc)
>
>We have compared the thermostabilities of Vent, Deep Vent and Pfu DNA
>polymerases.  Assessing the remaining polymerase activity as a function of 
>incubation time at 95 C-100 C, we find the Vent DNA polymerase has a half
>life at 95 C of 6.7 hours and at 100 C of 1.8 hours.  The Deep Vent DNA
>polymerase has a half life at 95 C of 23 hours and at 100 C of 8 hours.
>When we test Pfu DNA polymerase side-by-side with Vent and Deep Vent, we
>find its thermostability matches that of Deep Vent--not unexpected, since
>they are different species, but are the same genus.



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