MC1061/P3 minipreps...

wetsel_r at wetsel_r at
Tue Dec 8 06:24:08 EST 1992


Does anyone have any suggestions as to a good miniprep protocol for the MC1061/P3 cells?  They are not the most compliant cells when it comes to minipreps.
Standard alkaline-minis provide degraded DNA.  Promega mini-prep systems
fail completely and yeild NO DNA.  An altered alkaline mini prep protocol
(ala Maniatis) provides DNA but can not be digested.  Does anyone have any

Thanks in advance,

haviland at

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