MC1061/P3 minipreps...

wetsel_r at wums.wustl.edu wetsel_r at wums.wustl.edu
Tue Dec 8 06:24:08 EST 1992


Greetings:

Does anyone have any suggestions as to a good miniprep protocol for the MC1061/P3 cells?  They are not the most compliant cells when it comes to minipreps.
Standard alkaline-minis provide degraded DNA.  Promega mini-prep systems
fail completely and yeild NO DNA.  An altered alkaline mini prep protocol
(ala Maniatis) provides DNA but can not be digested.  Does anyone have any
suggestions?

Thanks in advance,

David
haviland at kids.wustl.edu



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