Immunopreciptation

vwalther at ulys.unil.ch vwalther at ulys.unil.ch
Tue Dec 8 09:00:34 EST 1992


In article <1992Dec4.181436.12469 at gserv1.dl.ac.uk>, skgtwcs at ucl.ac.uk (Wun Chey Sin) writes:
> Dear netters,
> I have difficulty in immunoprecipitating protein from PC12 cell lysate using polyclonal antibodies.  The antibodies definitely work on the western .  I have been doing DNA work and have no experience in protein work. Is there any things to take note 
> tricks.  What is the most likely reason?  (  The buffer contains the usual NaCL and inhibitors and 1% triton X)
> 
> Thanks
> Wun Chey

Your cell lysate extract is probably to crude to allow the antibodies to 
recognise your protein. Try to partially purify your protein before doing 
the immunoprecipitation.

V. Walther



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