vwalther at vwalther at
Tue Dec 8 09:00:34 EST 1992

In article <1992Dec4.181436.12469 at>, skgtwcs at (Wun Chey Sin) writes:
> Dear netters,
> I have difficulty in immunoprecipitating protein from PC12 cell lysate using polyclonal antibodies.  The antibodies definitely work on the western .  I have been doing DNA work and have no experience in protein work. Is there any things to take note 
> tricks.  What is the most likely reason?  (  The buffer contains the usual NaCL and inhibitors and 1% triton X)
> Thanks
> Wun Chey

Your cell lysate extract is probably to crude to allow the antibodies to 
recognise your protein. Try to partially purify your protein before doing 
the immunoprecipitation.

V. Walther

More information about the Methods mailing list