Immunopreciptation

DMEIER at mis.mcw.edu DMEIER at mis.mcw.edu
Tue Dec 8 11:41:37 EST 1992


...stuff deleted
>
>Thanks
>Wun Chey

>Your cell lysate extract is probably to crude to allow the antibodies to
>recognise your protein. Try to partially purify your protein before doing
>the immunoprecipitation.
>
>V. Walther

V., your comment is intriguing.  Do you think protein aggregation is 
blocking antibody from recognizing its epitope.  But in the presence of 
salt and Triton, I would have guessed aggregation is minimal.  Is there 
something I'm missing here? (the most likely scenario).
Thanks,
Dan Meier
DMEIER at mis.mcw.edu



More information about the Methods mailing list