DNA from Polyacrylamide & Protein Fusion and Purification System

[Michael CHENG]

  Hi! I was wondering if anyone out there knows of any company that sells a kit
for extracting DNA from polyacrylamide gels. According to Molecular Cloning
(2nd Edition) by Sambrook et al on page 6.46, there exists a "Crush and Soak"
method. However, the method is lengthy and inefficient. With so many kits
available for extracting DNA out of agarose gels, I haven't been able to find
one that's for use with polyacrylamide gels. Unfortunately, I must run my DNA
samples on PAGE. I would appreciate it if anyone could provide any info. or
even suggest a better method other than "Cursh and Soak".
  Secondly, has anyone used the Protein Fusion and Purification System made by
New England Lab.? I'll start using it in about 3 weeks and would like to know
if there's anything that I should watch out for? By the way, I think they
made some modification to the vectors compared to last year. Among one, they
changed the StuI site immediately after where Factor Xa cleaves to a XmnI
site?
  Thanks in advance.

Michael Cheng
Dept. of Biological Chemistry
Kyoto University

a52041 at sakura.kudpc.kyoto-u.ac.jp