Protein Fusion and Purification System

[Mark Bradley]

I read your very interesting comments on the modified MBP system.  We are
making extensive use of this system also and your comments are helpful to us.
we have one major problem; after Xa cleavage, the free MBP will not
rebind to the amylose column allowing purification of this away from 
the target protein - do you have any suggestions/experience in this area.

Mark Bradley