Agarose gel migration artifacts of PCR products.

Basavaraju Shankarappa bsh at MED.PITT.EDU
Wed Dec 9 19:07:34 EST 1992


Hi Netfolks:
	We have been trying to resolve the amplified product on a
agarose gel.  Usually we have no problem.  But in one case we tried
primers that contained lot of degeneracies.  When we run this product
on an agarose gel, there is a kind of smearing effect in the lanes.
This smear is clearly not caused by the presence of products of varied
sizes.  The lane looks like the DNA appears to have aggregated and
travels as a streak along the two edges of the lane.  We can also 
faintly see the band at the appropriate positions.
	We tried running the amplified product after diluting it and 
also added SDS.  But it does not seem to go away.  Has anybody else
seen this happen or has worked out a solution.  If I get a reply
that solves the problem, I will post on the net.
	Thanks 
Raj Shankarappa
Univ of Pittsburgh
bsh at med.pitt.edu



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