Bryan J. Maloney
jacobus at symphony.cc.purdue.edu
Wed Dec 9 20:50:17 EST 1992
A few possibilities:
The Maniatis method can cause problems with "indigestible" minipreps.
This is often due to alcohol remaining in the DNA. I try to be pretty
anal about rinsing with 70% EtOH and then drying to eliminate the
alcohol (yes, I know that many manuals warn about the dangers of overdrying,
but it's never seemed to be a problem for me). If you've got lots of funds
to spend, you could try a product from a company called Qiagen. It's a
column elution system which relies on alkaline lysis and then salt-elution.
We have pretty good success for moderate-scale preparations with the
columns we use.
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