Vent of Pfu - which is more HiFi ?

Stuart Brown browns at ccu.umanitoba.ca
Thu Dec 10 15:51:42 EST 1992


In article <axa12.111.723506746 at po.cwru.edu> axa12 at po.cwru.edu (Ashok Aiyar) writes:
>In article <9212032143.AA07769 at neb.com> kucera at neb.com writes:
>
>>>Has anyone out there compared the thermostable polymerases Vent and Pfu ?
>>>Any comments you may have on the RELATIVE merits of one over the other would
>>>be appreciated (eg length of synthesis products, stability, etc)
>>
>>We have compared the thermostabilities of Vent, Deep Vent and Pfu DNA
>>polymerases.  Assessing the remaining polymerase activity as a function of 
>>incubation time at 95 C-100 C, we find the Vent DNA polymerase has a half
>>life at 95 C of 6.7 hours and at 100 C of 1.8 hours.  The Deep Vent DNA
>>polymerase has a half life at 95 C of 23 hours and at 100 C of 8 hours.
>>When we test Pfu DNA polymerase side-by-side with Vent and Deep Vent, we
>>find its thermostability matches that of Deep Vent--not unexpected, since
>>they are different species, but are the same genus.
>
>From the perspective of a user who has tried all three enzymes (Vent, 
>DeepVent, and Pfu), I have had no trouble using Vent for amplification 
>reactions.  Deep Vent and Pfu have been more recalcitrant to performing
>well in amplification reactions.
>----------------------------------------------------------------------
>  Ashok Aiyar   Department of Biochemistry, CWRU   axa12 at po.cwru.edu

I have found good sequence of up to 800 bp from the primer using the
Vent polymerase.

	-Stu

-- 
Stuart M. Brown                             If you can remain cool when all 
U. of Manitoba, Dept. Plant Science         Around you are in panic,
Winnipeg, Manitoba, CANADA
browns at ccu.umanitoba.ca            Then you surely misunderstand the situation



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