Vent and exonuclease activity (was Re: PCR enzymes...)

Ashok Aiyar ashok at meds38956.cwru.edu
Thu Dec 10 19:41:55 EST 1992


In article <10DEC92.16070206 at wums.wustl.edu> wetsel_r at wums.wustl.edu writes:
>
>Through conversations with him, it is his experience that Vent polymerase (I
>believe this is the one) has more exonuclease activity that the original Taq.

Vent has a 3' - 5' exonuclease activity (proof reading) which Taq doesn't.  
Without raising any questions about the fidelity of delta-Taq,  Vent's
proof-reading ability is one the primary reasons we have switched enzymes
to Vent.

Ashok
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                              Ashok Aiyar 
                      Department of Biochemistry
                       CWRU School of Medicine
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