Sequencing Problem...

[wetsel_r at wums.wustl.edu]

Greetings netters:

This is probably a sequencing FAQ but here goes:  I have a technician in the
lab that can do a "single" load reasonably well.  The problem is when the
run is extended or even "double" loaded.  What we're getting is fuzzy
, sometimes doubled bands which are difficult to decipher.  Is the Urea going
off, temp too low/high, any suggestions would be appreciated...

OH! BTW - I should note that this is double-stranded sequencing with oligos
and using Sequenase.

Thanks in advance,

David
haviland at kids.wustl.edu