BOB AT COMB
belas at comb1.comb.umd.edu
Fri Dec 11 08:17:00 EST 1992
In article <10DEC92.15581742 at wums.wustl.edu>, wetsel_r at wums.wustl.edu writes...
>This is probably a sequencing FAQ but here goes: I have a technician in the
>lab that can do a "single" load reasonably well. The problem is when the
>run is extended or even "double" loaded. What we're getting is fuzzy
>, sometimes doubled bands which are difficult to decipher. Is the Urea going
>off, temp too low/high, any suggestions would be appreciated...
>OH! BTW - I should note that this is double-stranded sequencing with oligos
>and using Sequenase.
>Thanks in advance,
>haviland at kids.wustl.edu
Are you cleaning the wells before doing the second loading? I found
that if my tech forgets to do so we often end up with double bands or fuzzy
bands. Just blow out the accumulated urea using a syringe and needle. Hope
you solve your problem.
"Ignorance is a
-- Nicholas Ling
Bob (Belas at mbimail.umd.edu)
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