How to empower RNases?!

DMEIER at mis.mcw.edu DMEIER at mis.mcw.edu
Fri Dec 11 16:20:49 EST 1992


Hello All,l
  I'm sure this is a strange request to many of you who have labored 
long and hard to remove pesky RNAses, but I'd like to stabilize or 
increase RNase A and/or RNase T1.  I haven't been adding Mg to these 
incubations... Will Mg or Mn stabilize/increase RNase activity at 37 C
reaction temperatures, and will they stabilize RNAse A during long term
storage at - 20 C in 50% glycerol solutions.
  Thanks much.

  Dan
  DMEIER at MIS.MCW.EDU



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