IgM for affinity chromatography
nguyenhd at HERMES.BC.EDU
Fri Dec 11 15:14:22 EST 1992
I have purified IgM antibody from my culture media by Sephacryl 300. I have
also tried to couple this antibody to CNBr-activated Sepharose 4B. Antibody
binds to the beads, but it loses its activiy and leaks with the eluting buffer
(0.1M Glycine, pH 2.5). How do I get around these problems?
Thank you in advance.
Biology Dept., Boston College
NGUYENHD at hermes.bc.edu
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