Help: Panning

GARBRECHT fgarbrec at POST.ITS.MCW.EDU
Sat Dec 12 12:52:59 EST 1992


I'm not sure exactly what you are asking here, but (as an immunologist),
I have some experiance in the past using monoclonal antibodies to pan
for various lymphoid cell populations.  In particular, I have panned
for CD3, CD4 and CD8 bearing lymphocytes.  In general, panning is a
picky procedure and is dependent upon a number of factors, including
quality of plastic used, density of cell surface determinants, purity and
affinity of the antibody used, etc.  We have pretty much switched
entirely to immunomagnetic bead cell selection procedures.
Fred Garbrecht
Medical College of Wisconsin
fgarbrec at post.its.mcw.edu

On 12 Dec 1992, JnP wrote:

> Date: 12 Dec 92 01:23:30 GMT
> From: JnP <pnj at stein.u.washington.edu>
> To: methods-and-reagents at net.bio.net
> Subject: Help: Panning
> 
> Hi there, I would like to know how many surface proteins have people 
> successfully used for cell panning.  Could any expert on this provide 
> me with some information (also for panning in general)?  I will post 
> replies I get.  
> 
> Many thanks!
> 
> -Fred
> 





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