restriction map of EMBL4

afc at gnv.ifas.ufl.edu afc at gnv.ifas.ufl.edu
Mon Dec 14 11:54:59 EST 1992


In article <1gbna4INNmho at gaia.ucs.orst.edu>, chend at ucs.orst.edu (Donald Chen - Microbiology) writes:
> Hi:
> 
> The subject line says it all. I'd like to find a site where
> I can download either the sequence and / or the restriction
> map for the EMBL4 vector.  If there is a restriction map,
> hopefully it might include approximate sizes (based on the 
> linear map). I'm hoping to use the one / or more of the 
> following restriction enzymes: EcoRI, EcoRV, PstI.
> 
> Thanks for any help,
> 
> Don Chen
> Microbiology
> Oregon State University
> 
> chend at bionette.cgrb.orst.edu
> or
> chend at ucs.orst.edu

A fairly detailed map is published in Frishauf et al., Methods in Enzymology
volume 153, page 107.  This is a predicted map based on what they used to
assemble the vector, not on data.  (Map is of EMBL 3, so the polylinker is 
reversed).

Andrew Cockburn
USDA



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