3' overlaps in PCR
DMEIER at mis.mcw.edu
DMEIER at mis.mcw.edu
Mon Dec 14 18:04:06 EST 1992
Hello All PCR-ers,
Two potential primers have a 3 base overlap at the 3' end (GGC),
and have Tm's of 50.9 and 51.2 degrees C. If I remove a G from, the
forward primer for example, I get Tm's of 46.1 and 51.2 degrees C with
a 2 base overlap at the 3' end (GG). Is it worth sacrificing Tm
compatibility for reducing 3' overlapping? Incidently, the Tm of the
target cDNA is 86.8 degrees C.
Any comments will be appreciated.
Thanks.
Dan
DMEIER at MIS.MCW.EDU
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