PIPES

Nir Navot LCNAVOT at WEIZMANN.WEIZMANN.AC.IL
Tue Dec 15 07:17:57 EST 1992


A friend wishes to prepare a 1M solution of PIPES (buffer) at pH 6.4. He needs
it for working with RNA. Questions: 1. What should be used for its titration?,
NaOH / KOH or else? 2. Can PIPES be autoclaved ? Should it be autoclaved at all
.... any reason to think there would be RNAases there? 3. Can it be autoclaved
in the presence of DEPC?

Thanks in advance,
Nir



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