Acid washed sand

[LAKSHMAN at MAINE.MAINE.EDU]

We are trying to isolate nuclei from a fungus (R. Solani)
using a protocal which calls for "acid washed sand" to grind the
mycelium. Does anyone know how to acid wash sand (we are trying 50%
sulfuric acid overnight, is this appropriate)? Also why does it
need to be acid washed? Perhaps to remove any metal ions?

Thanks,
Dilip
DILIP LAKSHMAN                             LAKSHMAN at MAINE.MAINE.EDU
DEPT. PLANT BIOLOGY & PATHOLOGY
UNIV. MAINE AT ORONO
ORONO ME 04469