Plant protein isolation
bmcnulty at OAVAX.CSUCHICO.EDU
Thu Dec 17 11:28:37 EST 1992
I am working on an extraction of a plasma membrane protein from plant
roots. After separation in an aqueous phase system (dextran/PEG), I have
not had luck with phenol extraction of the protein from the membrane for
second dimension electrophoresis (the protein precipitates with methanol,
but transfer to the gel is poor). I am considering n-octyl-B-D-
glucopyranoside, but need suggestions as to concentration, incubation, and
mostly a method to remove the detergent for 2-D electrophoresis. Due to
funds, columns aren't really an option, and I have an extremely small
membrane pellet, so a series of detergent test ratios is hard to commit to!
Any suggestions would be greatly appreciated.
Thanks in advance.
//////// "Great spirits have always encountered violent opposition
( ) from mediocre minds."
\ @ @ / A. Einstein
Bob McNulty, CA. STATE UNIV., CHICO
BMCNULTY at OAVAX.CSUCHICO.EDU
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