Removing RNA from a mixture of protein and RNA

Basavaraju Shankarappa bsh at MED.PITT.EDU
Thu Dec 17 11:10:20 EST 1992


	Hi folks:

	Sometime back there was a question about removing RNA contamination
from a purified protein solution.  Among the many suggestions that were
provided including precipitating proteins selectively some were neat.

	There is one more way.  US biochemicals sells a large list of
immobilized enzymes and RNase A is one of those.  I think this would be
a good idea if you can purify the ribonucleotides away from the protein
or if it does not matter.
Raj Shankarappa
Univ of Pittsburgh
bsh at med.pitt.edu



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