Removing RNA from a mixture of protein and RNA

Christo Houbaviy lihouba at dapsas1.weizmann.ac.il
Sat Dec 19 15:27:04 EST 1992


In article <9212171610.AA02023 at miranda.med.pitt.edu> bsh at MED.PITT.EDU (Basavaraju Shankarappa) writes:
>
>	Hi folks:
>
>	Sometime back there was a question about removing RNA contamination
>from a purified protein solution.  Among the many suggestions that were
>provided including precipitating proteins selectively some were neat.
>
>	There is one more way.  US biochemicals sells a large list of
>immobilized enzymes and RNase A is one of those.  I think this would be
>a good idea if you can purify the ribonucleotides away from the protein
>or if it does not matter.

I think that this is a _great_ idea. Getting rid of the
ribonucleotides should be no problem - one can use gel filtration or
ultrafiltration (e.g. Centricon).

>Raj Shankarappa
>Univ of Pittsburgh
>bsh at med.pitt.edu

Regards,

Christo

--
Christo Houbaviy [lihouba at dapsas.weizmann.ac.il]
Department of Molecular Genetics and Virology
The Weizmann Institute of Science, Rehovot, Israel



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