gene synthesis

Ding Ming ming at ZEUS.AHABS.WISC.EDU
Mon Dec 21 11:13:29 EST 1992


Hi,

I isolated a protein recently and got the sequence by protein 
sequencing. Now I want synthesize the gene for this protein which 
only has 35 amino acids and send someone to have it expressed in 
E.coli. The problem now I am facing is which codon I should use for 
expression in E.coli. e.g., shall I use CUU, or CUG, or CUC etc. for 
lysine? Can someone give me a guid or just the references which I 
can look for by myself?

Thanks in advance.

-=ding=-



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    Ding Ming                     
    (608)265-3544                   ming at calshp.cals.wisc.edu
    Sweet Protein Section           ming at zeus.ahabs.wisc.edu
    Taste Research Lab               
    Department of Animal Health & Biomedical Sciences    
    University of Wisconsin-Madison, 1655 Linden Dr., Madison, WI53706      
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    Office Location: A215 Babcock Hall   HomePhone: 233-2159      
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