Acid washed sand
pamaga at biobase.aau.dk
Mon Dec 21 10:51:39 EST 1992
<LAKSHMAN at MAINE.MAINE.EDU> writes:
>We are trying to isolate nuclei from a fungus (R. Solani)
>using a protocal which calls for "acid washed sand" to grind the
>mycelium. Does anyone know how to acid wash sand (we are trying 50%
>sulfuric acid overnight, is this appropriate)? Also why does it
>need to be acid washed? Perhaps to remove any metal ions?
>DILIP LAKSHMAN LAKSHMAN at MAINE.MAINE.EDU
>DEPT. PLANT BIOLOGY & PATHOLOGY
>UNIV. MAINE AT ORONO
>ORONO ME 04469
In my "previous life" I worked with _Neurospora crassa_ and one of the
things essencial in preparing DNA (for example) is grinding with acid
washed sand. I don't have the exact protocol in my head (we only did
it about once a year, with enough sand to last for quite a while...),
but if you're really stuck, I can dig it up for you. This posting,
however, was more of a note on a different key: you don't mention
the SIZE (average diametre) of the sand you use... In my experience
this is an important parameter, so do check it!
Anything you may need, just shout.
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